Improving voltage-sensitive dye imaging: with a little help
from computational approaches.
Sandrine Chemla, Lyle Muller, Alexandre Reynaud, Sylvain
Takerkart, Alain Destexhe and Frédéric Chavane
Neurophotonics 4: 031215, 2017.
Voltage-sensitive dye imaging (VSDI) is a key neurophysiological
recording tool because it reaches brain scales that remain
inaccessible to other techniques. The development of this
technique from in vitro to the behaving nonhuman primate has only
been made possible thanks to the long-lasting, visionary work of
Amiram Grinvald. This work has opened new scientific perspectives
to the great benefit to the neuroscience community. However, this
unprecedented technique remains largely under-utilized, and many
future possibilities await for VSDI to reveal new functional
operations. One reason why this tool has not been used extensively
is the inherent complexity of the signal. For instance, the signal
reflects mainly the subthreshold neuronal population response and
is not linked to spiking activity in a straightforward manner.
Second, VSDI gives access to intracortical recurrent dynamics that
are intrinsically complex and therefore nontrivial to process.
Computational approaches are thus necessary to promote our
understanding and optimal use of this powerful technique. Here, we
review such approaches, from computational models to dissect the
mechanisms and origin of the recorded signal, to advanced signal
processing methods to unravel new neuronal interactions at
mesoscopic scale. Only a stronger development of interdisciplinary
approaches can bridge micro- to macroscales.
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