Oversampling method to extract excitatory and
inhibitory conductances from single-trial
membrane potential recordings.
Claude Bédard, Sébastien Béhuret, Charlotte
Deleuze, Thierry Bal and Alain Destexhe
J. Neurosci. Methods 210: 3-14, 2012.
Variations of excitatory and inhibitory conductances determine the
membrane potential (Vm) activity of neurons, as well as their spike
responses, and are thus of primary importance. Methods to estimate
these conductances require clamping the cell at several different
levels of Vm, thus making it impossible to estimate conductances
from "single trial'' Vm recordings. We present here a new method
that allows extracting estimates of the time course excitatory and
inhibitory conductances from single-trace Vm recordings. This
method is based on oversampling of the Vm. We test the method
numerically using models of increasing complexity. Finally, the
method is evaluated using controlled conductance injection in
cortical neurons in vitro using the dynamic-clamp technique.
This conductance extraction method should be very useful for future
in vivo applications.
This MATLAB code implements the method of conductance
extraction developed here, and also reproduces several figures of
the paper. The MATLAB code was written by Claude Bedard.
return to main page