Romain Brette and Alain Destexhe
In: Handbook of Neural Activity Measurement, Edited by
Brette R and Destexhe A, Cambridge University Press, Cambridge,
UK, pp. 44-91, 2012.
Intracellular electrophysiology is one the first method designed for
measuring neural activity. In this chapter, we review the various
techniques that are used to record the membrane potential
(current clamp), currents (voltage clamp) and conductances (more
specifically, synaptic conductances). The techniques are presented
from a modelling point of view, which emphasizes the limitations
and artifacts that apply to each technique. Models can be used to
improve the precision of experimental measurements, as shown by
the Active Electrode Compensation (AEC) technique. The models
are illustrated by numerical simulations that reproduce the
response of the cell, the electrode and the amplifier.
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